ecis zθ system (Applied BioPhysics)

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Applied BioPhysics ecis zθ system

A) hCMEC barrier resistance was assessed by <t>ECIS</t> <t>Zθ</t> to evaluate barrier integrity. Human CMEC were treated with 10µM Aβ40-Q22 (Q22), alone or in combination with 10 or 30µM 4ITP. B-C) Western blot analysis of occludin and oligomeric claudin-5 (normalized to actin, and plotted as % change of Ctrl), following 24h (B) and 48h (C) treatment. Data represents 3 individual experiments with 2 replicates per group, graphed as mean ± SEM. Statistical significance was evaluated by One-way ANOVA followed by Tukey post-hoc test. *P< 0.05, **P<0.01, ***P<0.001, ****P<0.0001

Ecis Zθ System, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 96/100, based on 636 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecis+z%CE%B8+system/ECIS+Z+Theta/bio_rxiv__64898__2026__03__16__711716-163-7-10
Average 96 stars, based on 636 article reviews

ecis zθ system - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "Mitochondrial carbonic anhydrase-VB inhibition rescues brain endothelial stress and memory in Alzheimer’s disease models"

Article Title: Mitochondrial carbonic anhydrase-VB inhibition rescues brain endothelial stress and memory in Alzheimer’s disease models

Journal: bioRxiv

doi: 10.64898/2026.03.16.711716

A) hCMEC barrier resistance was assessed by ECIS Zθ to evaluate barrier integrity. Human CMEC were treated with 10µM Aβ40-Q22 (Q22), alone or in combination with 10 or 30µM 4ITP. B-C) Western blot analysis of occludin and oligomeric claudin-5 (normalized to actin, and plotted as % change of Ctrl), following 24h (B) and 48h (C) treatment. Data represents 3 individual experiments with 2 replicates per group, graphed as mean ± SEM. Statistical significance was evaluated by One-way ANOVA followed by Tukey post-hoc test. *P< 0.05, **P<0.01, ***P<0.001, ****P<0.0001

Figure Legend Snippet: A) hCMEC barrier resistance was assessed by ECIS Zθ to evaluate barrier integrity. Human CMEC were treated with 10µM Aβ40-Q22 (Q22), alone or in combination with 10 or 30µM 4ITP. B-C) Western blot analysis of occludin and oligomeric claudin-5 (normalized to actin, and plotted as % change of Ctrl), following 24h (B) and 48h (C) treatment. Data represents 3 individual experiments with 2 replicates per group, graphed as mean ± SEM. Statistical significance was evaluated by One-way ANOVA followed by Tukey post-hoc test. *P< 0.05, **P<0.01, ***P<0.001, ****P<0.0001

Techniques Used: Western Blot

CA-VB KO in hCMEC protects from Aβ-induced apoptosis and BBB permeability . A) WB confirmed CA-VB absence in KO cells. B) DNA fragmentation, plotted as fold of change (FOC) of untreated control (ctrl) cells, measured by cell death ELISA. C) Representative IF images of active caspase-3/7 (green) and quantification to the right in CMEC treated with 25µM Q22 for 24 hours. Original magnification: 10x . D) IF of CA-VB KO vs WT hCMEC to detect mitochondrial membrane potential (Mito-tracker, red) as well as cytochrome C (Cyto C, green), in hCMEC challenged with Q22 for 16 hours. Zoom images of the merge signal at the bottom depict altered mitochondrial network with perinuclear mitochondria in Q22-treated hCMEC, but not in CA-VB KO cells. Original magnification:100x E) Mitochondrial H 2 O 2 measured with Amplex Red kit. F) Barrier resistance was measured over time with ECIS-Zθ in WT and CA-VB KO hCMEC treated or not with 10µM Q22. Data represents 3 individual experiments with 2 replicates each, graphed as mean ± SEM. Statistical significance was evaluated by Two-way ANOVA followed by Tukey post-hoc test. *P< 0.05, **P<0.01 ****P<0.0001

Figure Legend Snippet: CA-VB KO in hCMEC protects from Aβ-induced apoptosis and BBB permeability . A) WB confirmed CA-VB absence in KO cells. B) DNA fragmentation, plotted as fold of change (FOC) of untreated control (ctrl) cells, measured by cell death ELISA. C) Representative IF images of active caspase-3/7 (green) and quantification to the right in CMEC treated with 25µM Q22 for 24 hours. Original magnification: 10x . D) IF of CA-VB KO vs WT hCMEC to detect mitochondrial membrane potential (Mito-tracker, red) as well as cytochrome C (Cyto C, green), in hCMEC challenged with Q22 for 16 hours. Zoom images of the merge signal at the bottom depict altered mitochondrial network with perinuclear mitochondria in Q22-treated hCMEC, but not in CA-VB KO cells. Original magnification:100x E) Mitochondrial H 2 O 2 measured with Amplex Red kit. F) Barrier resistance was measured over time with ECIS-Zθ in WT and CA-VB KO hCMEC treated or not with 10µM Q22. Data represents 3 individual experiments with 2 replicates each, graphed as mean ± SEM. Statistical significance was evaluated by Two-way ANOVA followed by Tukey post-hoc test. *P< 0.05, **P<0.01 ****P<0.0001

Techniques Used: Permeability, Control, Enzyme-linked Immunosorbent Assay, Membrane

Electric Cell-substrate Impedance Sensing:

Article Title: Fibrillar tau alters cerebral endothelial cell metabolism, vascular inflammatory activation, and barrier function in vitro and in vivo
Article Snippet: .. We then tested the effects of these protofibrillar tau species on the endothelial barrier, using the ECIS ZΘ system (Applied Biophysics) to monitor trans‐endothelial electrical resistance (TEER) over time. ..

Article Title: Lack of Oxygen and/or Glucose Differentially Potentiates Aβ40 e22q - and Aβ42-Induced Cerebral Endothelial Cell Death, Barrier Dysfunction and Angiogenesis Impairment
Article Snippet: .. The ECIS Zθ system (Applied Biophysics, Troy, NY, USA) was utilized to assess HCMEC barrier integrity. ..

Article Title: Mitochondrial carbonic anhydrase-VB inhibition rescues brain endothelial stress and memory in Alzheimer’s disease models
Article Snippet: The same commercially available kit (Abcam 238538) was used for samples in vivo normalized to equal mg of protein. .. Trans-endothelial electrical resistance was measured using the ECIS ZΘ system (Applied Biophysics). .. All experiments were performed on 8-well ECIS (8WE10+, Applied Biophysics) 40-electrodes-gold plated arrays, pre-coated per the manufacturer’s instructions.

Article Title: LACK OF OXYGEN AND/OR GLUCOSE DIFFERENTIALLY POTENTIATES Aβ40 EQ22 - AND Aβ42-INDUCED CEREBRAL ENDOTHELIAL CELL DEATH, BARRIER DYFUNCTION AND ANGIOGENESIS IMPAIRMENT
Article Snippet: .. The ECIS Zθ system (Applied Biophysics) was utilized to assess HCMEC barrier integrity. ..

Article Title: Development and characterization of the mode-of-action of inhibitory and agonist peptides targeting the voltage-gated sodium channel SCN1B beta-subunit.
Article Snippet: Cardiac arrhythmia treatment is a clinical challenge necessitating safer and more effective therapies.. Recent studies have highlighted the role of the perinexus, an intercalated disc nanodomain enriched in voltage-gated sodium channels including both Nav1.5 and β1 subunits, adjacent to gap junctions.. These findings offer insights into action potential conduction in the heart.

Article Title: Lack of Oxygen and/or Glucose Differentially Potentiates Aβ40 e22q - and Aβ42-Induced Cerebral Endothelial Cell Death, Barrier Dysfunction and Angiogenesis Impairment.
Article Snippet: .. The ECIS Zθ system (Applied Biophysics, Troy, NY, USA) was utilized to assess HCMEC barrier integrity. ..

Article Title: Fibrillar tau alters cerebral endothelial cell metabolism, vascular inflammatory activation, and barrier function in vitro and in vivo.
Article Snippet: .. We then tested the effects of these protofibrillar tau species on the endothelial barrier, using the ECIS ZΘ system (Applied Biophysics) to monitor trans-endothelial electrical resistance (TEER) over time. ..

Expressing:

other:

Article Title: Astrocytes-Secreted WNT5B Disrupts the Blood-Brain Barrier Via ROR1/JNK/c-JUN Cascade During Meningitic Escherichia Coli Infection.
Article Snippet: The blood–brain barrier (BBB) is a complex structure that separates the central nervous system (CNS) from the peripheral blood circulation.. Effective communication between different cell types within the BBB is crucial for its proper functioning and maintenance of homeostasis.. In this study, we demonstrate that meningitic Escherichia coli (E. coli)-induced WNT5B plays a role in facilitating intercellular communication between astrocytes and brain microvascular endothelial cells (BMECs).

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